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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Mol Cancer Ther. 2015 Jul 3;14(9):2090–2102. doi: 10.1158/1535-7163.MCT-15-0017

Figure 6.

Figure 6

Expression of Thr150/Ser159 mutant SIRT3 inhibits clonogenic survival and increases tumor radiosensitivity. A, western blot of cytochrome c and caspase 3 cleavage in HCT-116 cells harboring wild type or Thr150/Ser159 mutant SIRT3 24 h after 5 Gy IR. B, apoptosis assay in HCT-116 cells harboring wild type or Thr150/Ser159 mutant SIRT3 upon sham or 5 Gy IR (n = 3, *, P < 0.05). C, Clonogenic survival rates were measured in above HCT-116 stable transfectants after irradiation with 5 and 10 Gy. Survival fractions normalized to mock sham controls were shown (n = 5, *, P < 0.05, **, P < 0.01). D, Clonogenic survival of HCT116 cells that are transfected with scrambled control or SIRT3 siRNA for 48 h, with or without radiation (n=3, *p<0.01). E, HCT-116 cells stably transfected with mock, wild type or mutant SIRT3 were inoculated in mice and when tumor reached ~ 0.3 cm3, 5 Gy IR was delivered locally to tumors with surrounding tissue shielded. a, tumor volumes were measured post-irradiation every 3 days (mean ± SD; n = 6). b, Representative photographs of surgically removed tumors at the end of experiments were shown (see also Fig. S6).