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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Antiviral Res. 2015 Aug 4;122:82–90. doi: 10.1016/j.antiviral.2015.08.001

Fig. 1. Co-immunization with M2e5x protein and split vaccines induces M2e- and virus-specific IgG and IgG isotype antibody responses.

Fig. 1

BALB/c mice (n = 8) were immunized with M2e5x protein (M2e5x) or H3N2 influenza split vaccine (Split) alone or M2e5x protein plus split vaccine (M2e5x.Split). All groups of vaccines were immunized with AS04 adjuvant. Blood samples were collected at 3 weeks after second boost. (A–C) M2e-specific total IgG (A), IgG1 (B), and IgG2a (C) antibody. (D–F) Vaccine-specific total IgG (D), IgG1 (E), and IgG2a (F) antibody. ELISA was performed with serially diluted serum samples for antibodies specific for M2e peptide (A–C) or H3N2 influenza split vaccine (D-F) (NYMC X-187). Error bars indicates mean ± SEM. Statistical significance was determined by 2-way ANOVA. Asterisks indicate significant differences (*p < 0.05) compared with the results in the M2e5x group.