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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Antiviral Res. 2015 Aug 4;122:82–90. doi: 10.1016/j.antiviral.2015.08.001

Fig. 4. M2e5x.Split vaccine enhances M2e-specific cytokine and antibody secreting cellular immune responses.

Fig. 4

(A) IFN-γ-secreting cells in lungs (n = 3). (B) IFN-γ-secreting cells in spleens (n = 3). Lung cells and splenocytes were isolated from mice at day 5 p.i. Cytokine-producing cell spots were counted by ELISPOT reader. Spleen (C) or bone marrow (D) cells were isolated from mice at day 5 p.i. and were incubated with M2e5x protein for in vitro stimulation. Culture supernatants were harvested after 1 or 5 days of culture. M2e-specific IgG levels were determined by ELISA. Data represent mean ± SEM. Statistical significance was determined by 1-way ANOVA. Plus sings indicate significant difference (++p < 0.01, and +++p < 0.001) compared with the results in the M2e5x group. Pounds indicate significant differences (#p < 0.05, ##p < 0.01, and ###p < 0.001) compared with the results in the Split group. Asterisks indicate significant differences (*p < 0.05, **p < 0.01, and ***p < 0.001) compared with the results in the Naive group.