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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Nat Med. 2015 Aug 17;21(9):1054–1059. doi: 10.1038/nm.3924

Figure 2.

Figure 2

Lowering WAVE1 expression reduces Aβ. (a–g) N2a/APPswe.PS1ΔE9 cells were transfected with control siRNA plus control plasmid (Control), Wasf1 siRNA plus control plasmid (Wasf1 siRNA), or Wasf1 siRNA plus siRNA-resistant plasmid for WAVE1 (WAVE1 add-back). WAVE1 (a, n = 9), Aβ40 (b, n = 6), Aβ42 (c, n = 6), total APP (f, n = 4) and actin were measured in cell lysates. Surface APP was measured by a biotinylation assay (d, n = 6). Soluble ectodomain of APP (sAPPβ) produced by β-secretase (e, n = 4), and sAPPα, a product of α-secretase (g, n = 4), were measured in the medium. Data represent mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001, two-tailed t-test. (h, i) The levels of Aβ40 (h) and Aβ42 (i) were measured in mouse brains of 4–5 month-old 2xTg AD male mice (tg/+) harboring Wasf1+/+ (n = 13), Wasf1+/– (n = 13) or Wasf1–/– (n = 6). Data represent means ± SEM. *P < 0.05 and **P < 0.01, one-way analysis of variance (ANOVA), Dunnett's post hoc test.