Table 1.

Cell death and STAT1 phosphorylation following a range of cisplatin and cetuximab doses in two cell lines. Cells were treated with the indicated drug doses for 24 hours to assess cell death or 8 hours to assess STAT1 phosphorylation. Mean cell death according to these assays was 2% or less for untreated cells. Levels of phosphorylated STAT1 are reported as fold change in median fluorescence intensity by flow cytometry, compared with untreated cells. Data are mean ± SEM.

Cell Line	Drug	Dose (μg/ml)	% Apoptotic Cells (Annexin V positive)	% Apoptotic Cells (TUNEL positive)	p-S727-STAT1 (Fold Change vs. Untreated)	p-Y701-STAT1 (Fold Change vs. Untreated)
JHU029	Cisplatin	2	20 ± 1.66	9.07 ± 1.88	0.82 ± 0.16	1.22 ± 0.27
		6	22.07 ± 0.45	22.6 ± 4.92	1.42 ± 0.27	1.05 ± 0.13
		12	45.7 ± 1.56	43.5 ± 6.47	1.94 ± 0.58	0.96 ± 0.04
	Cetuximab	1	53.37 ± 4.86	26.3 ± 2.77	0.81 ± 0.07	1.41 ± 0.04
		10	60.57 ± 1.27	28.4 ± 1.07	0.37 ± 0.16	0.80 ± 0.24
		20	62.8 ± 3.59	34.7 ± 1.31	0.63 ± 0.05	0.98 ± 0.11
PCI13	Cisplatin	2	30.36 ± 9.16	27.0 ± 0.44	1.52 ± 0.04	1.00 ± 0.02
		6	44.8 ± 2.28	67.5 ± 4.50	1.75 ± 0.07	0.89 ± 0.03
		12	46.4 ± 2.32	73.4 ± 7.14	1.35 ± 0.08	0.80 ± 0.02
	Cetuximab	1	18.67 ± 1.16	9.9 ± 2.33	1.25 ± 0.41	1.04 ± 0.05
		10	29.07 ± 1.19	14.5 ± 5.55	1.15 ± 0.09	1.16 ± 0.05
		20	27.2 ± 2.21	17.5 ± 0.44	1.63 ± 0.35	1.03 ± 0.02