Fig. 4.

Analysis of the 53D1 chiA-like ORF and encoded protein. a DNA level. Predicted RNA polymerase interaction region (shadowed), −35 and −10 regions, mRNA start site, Shine–Dalgarno region, start, and stop codon are marked. The presence of these elements was inferred by using BProm (SoftBerry) and the RBS Calculator. b Protein level. Motifs identified within the 396-amino-acid long 53D1 polypeptide chain include a family-18 GH catalytic domain (amino acids 16–393) and a chitin insertion domain (CID, 290–394). The conserved consensus sequences within the catalytic domain and the CID are highlighted, with the corresponding amino acid positions. Conserved residues essential for catalysis are indicated by asterisks; the Trp presumably involved in the processivity of the chitinase is indicated by section sign. Sequence analysis was performed using InterPro Scan and Prosite