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. Author manuscript; available in PMC: 2016 Sep 15.
Published in final edited form as: J Immunol. 2015 Aug 14;195(6):2861–2869. doi: 10.4049/jimmunol.1500877

Figure 5. CX3CR1 gene promoter activity is affected by DNA methylation.

Figure 5

(A) Schematic presentation of the CX3CR1 gene promoter region inserted into the luciferase reporter gene construct (pGL3-CX3CR1-P). (B) The pGL3-CX3CR1-P and pGL3-basic vector were transiently transfected into 293 T cells with the pRL-TK vector (Renilla luciferase control reporter vector) as an internal control. Promoter activity is expressed as relative light units (RLU) to the pGL3-basic vector of which was set as 1. (C) pGL3-CX3CR1-P was methylated using SssI methylase (see details in Materials and Methods). Unmethylaed and methylated pGL3-CX3CR1-P were transfected into 293T cells with pRL-TK vector. Promoter activity is expressed as RLU to the unmethylated pGL3-CX3CR1-P of which was set as 1. Bars and error bars indicate the mean and SD, respectively, (n = 6 independent experiments performed in triplicates) (B, C).