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. 2014 Dec 27;50(9):975–983. doi: 10.1007/s00535-014-1030-3

Fig. 3.

Fig. 3

The expression of CD44 in PDAC (HS766T and Panc-1) and HCC (HepG2 and HuH7) cell lines. Cells were cultured in 20 % O2 or 1 % O2 for 24 h, and the expression of the CSC marker CD44 was then estimated by flow cytometry and compared to cancer stem cell markers by hypoxic stimulation. The expression of CD44 was much higher in the PDAC cell lines compared to the HCC cell lines, but the 24-h incubation in 1 % O2 did not change the expression of CD44 in any of the cell lines. The green line shows the expression of CD44 in hypoxia (1 % O2 for 24 h) and the purple area shows it in normoxia (20 % O2 for 24 h)