Fig. 6.
Knockdown of PHD3 in the PDAC cell line HS766T. a Knockdown of PHD3 was done by siRNA, and the expression levels of PHD3 and HIF-1α were estimated by Western blot. PHD3 knockdown did not change the expression of HIF-1α when the cells were cultured in 20 % O2, but the expression of HIF-1α was clearly enhanced when the cells were cultured in 1 % O2. b The semiquantitative data in HIF-1α are shown. c Hypoxic stimulation by 1 % O2 significantly enhanced the VEGF secretion from HS766T cells treated by siPHD3 (p < 0.05)