Table 1.
Sacrifice day p.i. | Prairie dog # | Max. # of cutaneous lesions observed | OPXV antibodies detected | Neutralizing antibodies detected | Clinical symptoms | Gross pathology | Poxvirus IHC | Viable virus (#positive/#tested) |
---|---|---|---|---|---|---|---|---|
2 | PD9 | 0 | Neg. | Neg. | N.O. | N.O. | N.E. | 0/28 |
PD10 | 0 | Neg. | Neg. | N.O. | N.O. | N.E. | 0/28 | |
| ||||||||
4 | PD11 | 0 | Neg. | Neg. | N.O. | N.O. | N.O. | 0/28 |
PD12 | 0 | Neg. | Neg. | N.O. | N.O. | N.O. | 0/28 | |
| ||||||||
6 | PD13 | 0 | Neg. | Neg. | N.O. | N.O. | N.O. | 10/28 |
PD14 | 0 | Neg. | Neg. | N.O. | N.O. | N.O. | 10/28 | |
| ||||||||
9 | PD15 | 0 | Neg. | + | R.D. | Pale kidneys | LN, epithelial, dermis | 15/28 |
PD16 | 0 | Neg. | Neg. | R.D. | N.O. | LN, spleen | 16/28 | |
| ||||||||
12 | PD17 | 20 | + | N.E. | Inappetence | Lymphadenopathy, inflamed oviducts | Buccal/caudal pharyngeal mucosa, uterus, mesenchymal cells (mononuclear inflammatory cells/reactive fibroblasts) | 21/28 |
PD18 | 12 | + | N.E. | Inappetence | Lymphadenopathy | Epithelial, tonsil, fibroblasts | 10/28 | |
PD21† | 3 | N.E. | N.E. | Inappetence, nasal discharge | Hemorrhage of uterine horns and adipose tissue associated with the round ligament, hyperemic areas associated with the ovaries and inflamed oviducts, and hepatomegaly | All tissues positive for antigen except for brain | 26/28 | |
| ||||||||
17 | PD19 | >20 | + | + | Inappetence | Lymphadenopathy, hemorrhagic foci in the adipose tissue associated with lymph nodes and multiple, mild foci of serosal hemorrhage on the lungs | Peritesticular adipose tissue | 14/28 |
| ||||||||
24 | PD20 | 5 | + | N.E. | N.O. | N.O. | N.E. | 0/28 |
PD22 | 20 | + | N.E. | N.O. | Pericarditis | N.E. | 1/28 |
Groups of prairie dogs were serially sacrificed (days 2, 4, 6, 9, 12, 17, and 24) following challenge (n = 14). Animals were observed for clinical symptoms, antibody production, and lesion presentation. Gross observations were recorded and postmortem samples were evaluated for presence of virus and histopathological changes. R.D.: respiratory depression under anesthesia; N.O.: nothing observed; N.E. not examined.
†: unscheduled death.