Figure 2.

Effects of bleaching on neurobiotin injected brains. (A) Light microscopic image of a neurobiotin injected brain of D. elpenor bleached with hydrogen peroxide for 2 h. (B–D) Single optical sections from a confocal image stack, showing neurobiotin injected neurons in the optic lobe imaged with a 10x objective (B), as well as with a 25× objective in the lobula plate and medulla (C), and the lamina (dotted outlines, (D)). (B) and (C) are taken from the brain shown in (A). Note that neurites located underneath the stemmata (dotted region in (A–C)) are well-visible when imaged with the high magnification objective. Scale bars: 200 μm (A,B), 100 μm (C), 50 μm (D).