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. 2015 Sep 7;6:912. doi: 10.3389/fmicb.2015.00912

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Copyright © 2015 Iwai, Hori, Sasaki-Sekimoto, Shimojima and Ohta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in the TAG content of CrDGTT4-overexpressing cells in response to P starvation. Nannochloropsis cells were transformed with pCrSQD2-CrDGTT4 to overexpress CrDGTT4. Cells cultured to a logarithmic phase under standard conditions were then inoculated into P-starved medium and cultured for 4 days. Three transformants, the empty vector control (control) and the wild type (WT) are shown. Values represent the mean ± SD from four independent replicates. Asterisks indicate a statistically significant difference compared with the empty vector control based on a two-tailed Student's t-test (^*P < 0.05 and ^**P < 0.01).