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. 2015 Sep 7;6:702. doi: 10.3389/fpls.2015.00702

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Copyright © 2015 Hernández-Sánchez, Maruri-López, Ferrando, Carbonell, Graether and Jiménez-Bremont.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The OpsDHN1 histidine-rich motif is required for its nuclear subcellular localization. (A) Schematic representation of the pMDC43-OpsDHN1-ΔHis construct. (B) Schematic representation of BiFC pYFN43- and pYFC43- OpsDHN1-ΔHis vectors. (C) Cytoplasm localization of GFP::OpsDHN1-ΔHis translational fusion in N. benthamiana leaves. White arrowheads indicate cytosolic and nuclear compartments. (D) BiFC assay of OpsDHN1-ΔHis version. Transient fluorescent expression was analyzed by laser-scanning confocal microscopy. From left to right: the GFP and DAPI fluorescence spectrum, bright field, chlorophyll fluorescence and overlay signals. The S-segment and K-segments are depicted as dark-gray box and black boxes, respectively. The deleted histidine-rich motif is represented by an open triangle. The scale bar corresponds to 10 μm.