Figure 5.

The OpsDHN1 S-segment is involved in its nuclear location. (A) Schematic representation of the pMDC43-OpsDHN1-ΔSer construct. Fluorescent visualization of (B) GFP::OpsDHN1-ΔSer, (C) GFP::OpsDHN1, (D) GFP::OpsDHN1-ΔHis translational fusions in N. benthamiana leaves. White arrowheads indicate cytosol and nuclear signals. The fluorescence was examined by laser-scanning confocal microscopy. From left to right: the GFP and DAPI fluorescence spectrum, bright field, chlorophyll fluorescence and overlay signals. The K-segments and histidine-rich motif are depicted as black and light-gray boxes, respectively. The deleted S-segment is represented by open triangle. The scale bar corresponds to 10 μm.