Table 2.

Oligonucleotide primers and conditions used in this study for quantitative PCR analysis.

Category	Target gene	Forward primer (nM)	Reverse primer (nM)	Positive control	Ta	Reference
Calvin Benton Bassham	RuBisCO form II	cbbM591F (200) TTC TGG CTG GGB GGH GAY TTY ATY AAR AAY GAC GA	cbbM918R (200) CCG TGR CCR GCV CGR TGG TAR TG	Thiomicrospira crunogena (DMS No. 12353)	55	Campbell and Cary (2004)
rTCA	ATP citrate lyase	aclB892F (200) TGG ACM ATG GTD GCY GGK GGT	aclB1204R (200) GTT GGG GCC RCC WCK KCK NAC	Sulfurovum denitrificans (DMS No. 1251)	55	Takai et al. (2005)
Methano genesis	Methyl CoM reductase	qmcrAF-alt (150) GAR GAC CAC TTY GGH GGT TC	ML-R (200) TTC ATT GCR TAG TTW GGR TAG TT	Methanosarcina acetivorans, Methanococcus jannaschii	55	Luton et al. (2002), Ver Eecke et al. (2012)
Bacteria	16S rRNA	Bact1369F (1000) GTT GGG GCC RCC WCK KCK NAC	Prok1541R (1000) CGG TGA ATA TGC CCC TGC	Arcobacter nitrofigulis	59	Suzuki et al. (2001)
Archaea	16S rRNA	Arch1-1369F (500) CGG TGA ATA CGT CCC TGC + Arch2-1369F (500) CGG TGA ATA TGC CCC TGC	Prok1541R (1000) CGG TGA ATA TGC CCC TGC	Ferroplasma acidarmonas Fer1	59	Suzuki et al. (2001)
Archaea	16S rRNA	Arch349F (500) GYG CAS CAG KCG MGA AW	Arch806R (500) GGA CTA CVS GGG TAT CTA AT	Ferroplasma acidarmonas Fer1	54	Takai and Horikoshi (2000)

Ta, annealing temperature during QPCR reaction.