. 2015 Jul 10;14:101. doi: 10.1186/s12934-015-0292-7

Table 1.

Overview of dynamic fed-batch experiments

Experiment	Substrate	Feeding strategy based on q_s (g g⁻¹ h⁻¹)	Goals
FB1	Glycerol	Batch on glycerol–step-wise decrease of q_{s glycerol}: 0.338–0.063–0.054–0.029–0.014–0.005 g g⁻¹ h⁻¹	Determination of strain physiological parameters Characterization of recombinant expression profile
FB2	Glycerol	Batch on glycerol–repression phase on glycerol (q_s = 0.29 g g⁻¹ h⁻¹)–de-repression phase on glycerol (q_s = 0.035 g g⁻¹ h⁻¹)	Mimic optimized industrial process comprising of three phases
FB3	Sorbitol	Batch on sorbitol–step-wise increase of q_{s sorbitol}: 0.033–0.060–0.127–0.176–0.197 g g⁻¹ h⁻¹	Determination of strain physiological parameters Characterization of recombinant expression profile
FB3	Sorbitol	Glycerol pulse at a final concentration of 10 g L⁻¹ at highest q_{s sorbitol}	q_s in presence of both substrates
FB4	Mixed feed	Batch on glycerol–repression phase on glycerol (q_s = 0.33 g g⁻¹ h⁻¹)–de-repression phase on glycerol (q_s = 0.054 g g⁻¹ h⁻¹)–mixed-feed: glycerol (q_s = 0.054 g g⁻¹ h⁻¹) and sorbitol (q_s = 0.070 g g⁻¹ h⁻¹)–mixed-feed: glycerol (q_s = 0.026 g g⁻¹ h⁻¹) and sorbitol (q_s = 0.015 g g⁻¹ h⁻¹)	Analyze physiology and productivity in mixed feed environment
FB5	Mixed feed	batch on glycerol– repression phase on glycerol (q_s = 0.33 g⁻¹ h⁻¹)–mixed-feed: glycerol (q_s = 0.040 g⁻¹ h⁻¹) and sorbitol (q_s = 0.055 g g⁻¹ h⁻¹)	Verify increased STY in mixed feed environment
FB6	Mixed feed	batch on glycerol–repression phase on glycerol (q_s = 0.23 g g⁻¹ h⁻¹)–mixed-feed: glycerol (q_s = 0.026 g g⁻¹ h⁻¹) and sorbitol (q_s = 0.027 g g⁻¹ h⁻¹)–mixed-feed: glycerol (q_s = 0.026 g g⁻¹ h⁻¹) and sorbitol (q_s = 0.061 g g⁻¹ h⁻¹)	Verify increased STY in mixed feed environment Identification of q_s _glycerol/q_{s sorbitol} ratio allowing highest productivity and STY