Table 1. The most abundant and enriched CAZy families (top-15) in the RWS and TWS metagenomes (compared with FS1).
CAZy family | Metagenomes | Most common activities (EC Number) | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
FS1 | 1W | 3W | 10W | 1T | 3T | 10T | |||||||||
Log | RA | Log | RA | Log | RA | Log | RA | Log | RA | Log | RA | Log | RA | ||
PL17 | 0 | 0,017 | 1,135 | 0,236 | 1,071 | 0,203 | 1,122c | 0,229 | 1,267 | 0,320 | 1,304 | 0,348 | 1,234c | 0,296 | Alginate lyase (EC 4.2.2.3); Oligoalginate lyase (EC 4.2.2.-) |
CBM50 | 0 | 0,742 | 0,342 | 1,633 | 0,293 | 1,458 | 0,427a | 1,983 | 0,548 | 2,622 | 0,426 | 1,980 | 0,228b | 1,254 | Carbohydrate-Binding Module (peptidoglycan and chitin binding module) |
GT8 | 0 | 0,009 | 0,826 | 0,058 | 0,969 | 0,080 | 1,367a | 0,201 | 0,980 | 0,082 | 0,263 | 0,016 | 0,757b | 0,049 | Lipopolysaccharide galacto(gluco)syltransferase; Homogalacturonan galacturonosyltransferase; Xylan glucuronyltransferase (EC 2.4.1.-) |
GH117 | 0 | 0,009 | 1,593 | 0,338 | 1,513 | 0,281 | 1,528c | 0,291 | 0,883 | 0,066 | 1,564 | 0,316 | 1,798a | 0,542 | Neoagarooligosaccharide hydrolase (EC 3.2.1.-) |
GH92 | 0 | 0,311 | 1,060 | 3,564 | 0,978 | 2,955 | 0,861b | 2,254 | 0,465 | 0,906 | 0,980 | 2,967 | 1,197a | 4,895 | Alpha-mannosidase (EC 3.2.1.24) |
GH50 | 0 | 0,009 | 1,159 | 0,125 | 0,694 | 0,043 | 0,808b | 0,055 | 1,306 | 0,175 | 1,144 | 0,120 | 1,264b | 0,158 | Beta-agarase (EC 3.2.1.81) |
GH95 | 0 | 0,423 | 0,663 | 1,944 | 0,606 | 1,707 | 0,649c | 1,886 | 0,181 | 0,642 | 0,636 | 1,831 | 0,797a | 2,648 | Alpha-1,2-L-fucosidase (EC 3.2.1.63); Alpha-L-fucosidase (EC 3.2.1.51) |
GH20 | 0 | 0,570 | 0,523 | 1,900 | 0,462 | 1,652 | 0,384b | 1,380 | 0,078 | 0,682 | 0,405 | 1,449 | 0,617a | 2,359 | Beta-hexosaminidase (EC 3.2.1.52); Lacto-N-biosidase (EC 3.2.1.140) |
GH31 | 0 | 0,768 | 0,376 | 1,824 | 0,424 | 2,038 | 0,444a | 2,136 | 0,446 | 2,148 | 0,502 | 2,439 | 0,492c | 2,384 | Alpha-glucosidase (EC 3.2.1.20); Alpha-xylosidase (EC 3.2.1.177); Alpha-mannosidase (EC 3.2.1.24) |
GH2 | 0 | 1,839 | 0,336 | 3,987 | 0,410 | 4,722 | 0,417a | 4,806 | 0,245 | 3,235 | 0,334 | 3,963 | 0,409a | 4,715 | *Beta-galactosidase (EC 3.2.1.23); *Beta-mannosidase (EC 3.2.1.25); Beta-glucuronidase (EC 3.2.1.31); Alpha-L-arabinofuranosidase (EC 3.2.1.55) |
GH43 | 0 | 1,364 | 0,378 | 3,257 | 0,411 | 3,517 | 0,362c | 3,141 | 0,210 | 2,214 | 0,366 | 3,166 | 0,348a | 3,039 | *Beta-xylosidase (EC 3.2.1.37); Alpha-L-arabinofuranosidase (EC 3.2.1.55); Xylanase (EC 3.2.1.8); Galactan 1,3-beta-galactosidase (EC 3.2.1.145) |
GH1 | 0 | 0,656 | 0,260 | 1,192 | 0,424 | 1,740 | 0,457a | 1,879 | 0,665 | 3,034 | 0,552 | 2,341 | 0,320b | 1,370 | *Beta-glucosidase (EC 3.2.1.21); Beta-galactosidase (EC 3.2.1.23); Beta-mannosidase (EC 3.2.1.25); Beta-glucuronidase (EC 3.2.1.31); Beta-xylosidase (EC 3.2.1.37); Beta-D-fucosidase (EC 3.2.1.38) |
GH29 | 0 | 0,716 | 0,326 | 1,517 | 0,349 | 1,602 | 0,342c | 1,574 | −0,112 | 0,553 | 0,331 | 1,534 | 0,536a | 2,461 | Alpha-L-fucosidase (EC 3.2.1.51); Alpha-1,3/1,4-L-fucosidase (EC 3.2.1.111) |
GH3 | 0 | 2,762 | 0,177 | 4,156 | 0,160 | 3,996 | 0,158c | 3,974 | 0,139 | 3,804 | 0,083 | 3,343 | 0,035b | 2,993 | Beta-glucosidase (EC 3.2.1.21); Xylan 1,4-beta-xylosidase (EC 3.2.1.37); Beta-glucosylceramidase (EC 3.2.1.45); Beta-N-acetylhexosaminidase (EC 3.2.1.52); Alpha-L-arabinofuranosidase (EC 3.2.1.55) |
GH13 | 0 | 7,242 | −0,245 | 4,120 | −0,258 | 4,001 | −0,257c | 4,008 | −0,090 | 5,893 | −0,099 | 5,760 | −0,264b | 3,940 | Alpha-amylase (EC 3.2.1.1); Pullulanase (EC 3.2.1.41) |
Log: Log10 X-fold increase in relative abundance.
RA: Relative abundance.
Negative values are subfamilies deselected comparatively with the FS1.
*Detected enzymatic activity in the secretome of both consortia at transfer-10 (Jimenez et al., 2014b).
aEnriched along the transfers (selecting).
bDepleted along the transfers (deselecting).
cNeutral enrichment along the transfers.