N. crassa calcium sensor-1 gene, cse-1. The three SNPs (arrows) are shown that associated strongly with variation in the phenotype of germling signaling in a genome-wide association study (GWAS) (Palma-Guerrero et al. 2013). Although there were SNPs in the exons, they did not associate, demonstrating the precision that can be achieved using GWAS in a small, panmictic population of an oblilgately outbreeding fungus. Introns are thin lines, exons are thick lines, and 5′ and 3′ untranslated regions are of intermediate thickness. (From Palma-Guerrero et al. 2013; reprinted, with permission, from the author.)