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. 2015 Sep;22(9):472–493. doi: 10.1101/lm.037267.114

Figure 7.

Figure 7.

Dorsal hippocampal mGluR1a activation is essential in ovariectomized mice for ERα and ERβ to enhance object recognition and object placement memory consolidation, and to increase dorsal hippocampal ERK phosphorylation. (A,B) Immediate post-training infusion of PPT (0.2 pg) or DPN (20 pg) into the dorsal third ventricle significantly increased the time spent with the novel object (A) and moved object (B) relative to chance (dashed line at 15 sec, **P < 0.01); these effects were blocked by dorsal hippocampal infusion of the mGluR1a antagonist LY367385 (10 pg/hemisphere). Bars represent the mean ± SEM time spent with each object. (C) Five min after dorsal third ventricle infusion, PPT and DPN significantly increased phospho-p42 ERK levels (*P < 0.05; **P < 0.01 relative to vehicle); this effect was completely abolished by dorsal hippocampal infusion of LY367385. Bars represent mean ± SEM % change from vehicle. Phosphorylated ERK levels were normalized to total ERK. Insets are representative Western blots of phosphorylated and total protein. (Reprinted from Boulware et al. 2013).