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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Dev Biol. 2015 Jun 24;405(1):1–9. doi: 10.1016/j.ydbio.2015.06.005

Figure 2. Knockdown of sh3bgr caused severe defects in somite development.

Figure 2

A-C. Microinjection of sh3bgr antisense morpholino (Sh3bgr-MO; 50ng per embryo) disrupted normal somite development. The somite boundaries were labeled with anti-integrin-β1 antibody (Red) and the muscle fibers were stained with the myosin heavy chain (MHC) antibody (Green) at Stage 33. D. The muscle phenotypes were categorized according to the severity. Weak; the chevron pattern is partially disrupted, Severe; the chevron pattern is completely lost. Very severe; the somatic tissue is severely hypomorphic. The muscle defects in the morphants were recovered by co-injection with 1.5ng of sh3bgr mRNA. The numbers in the graph are the percentage of the embryos. The numbers of embryos analyzed: Control-13, sh3bgr morphants-26, Rescue-35. E-E’. Sh3bgr localizes to the muscle fibers. 150ng of Sh3bgr-flag mRNA was injected into Stage 2 embryos. Stage 33 embryos were fixed and the coronal sections of somitic tissues were stained for MHC (Red) and Sh3bgr-flag (Green). F-F’. Sh3bgr-flag fusion protein localized to the Z-line in sarcomeres. Sh3bgr-flag (Green) was co-immunostained with α-actinin (Red). The yellow line indicates a somitic boundary. Scale bar = 5μm