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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Dev Biol. 2015 Jun 24;405(1):1–9. doi: 10.1016/j.ydbio.2015.06.005

Figure 4. Overexpression of Sh3bgr caused abnormal sarcomere formation and increased heart volume.

Figure 4

A-C. Stage 33 embryos were fixed and the coronal sections of somitic tissues were stained for myomesin (Red) and MHC (Green). Overexpression of sh3bgr (2ng of Sh3bgr-flag mRNA) caused abnormally discontinuous thick filaments and mislocalizaiton of myomesin. D-F. Overexpression of Sh3bgr increased the heart volumes. The embryonic hearts were stained with anti-MHC antibody and the volumes of Stage 45 embryos were measured using the Imaris program. D’-F’. The surface views of 3D confocal images in (D-F) rendered using the Imaris program. The heart volume was plotted in (G). The numbers of embryos analyzed: Control-13, sh3bgr morphants-13, Rescue-13. Scale bar = 5μm