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. 2015 Aug 15;29(16):1707–1720. doi: 10.1101/gad.262568.115

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© 2015 Caserta et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — PTEN^FV is depleted in the nucleus. (A) Cell compartment fractionation of MEFs with the indicated genotypes. Protein lysates from each fraction were immunoblotted and probed with compartment-specific markers (Histone 4, HDAC1, EGFR, and PKCa) and a PTEN-specific antibody. (B) Immunohistochemistry (IHC) of sections from the indicated organs of E15.5 Pten^+/+ and Pten^FV/FV embryos using a PTEN-specific antibody; IHC stained sections were counterstained with hematoxylin. The boxes in the top panels indicate regions shown at high magnification in the panels directly below. Bars: low magnification, 200 µm; high magnification, 50 µm. (C) Confocal images showing PTEN localization (green) in E15.5 embryonic tissues that were probed with the PTEN-specific antibody. Tissue sections were counterstained with DAPI (blue) to demarcate nuclei. Bar, 50 µm.