Extended Data Table 2 . Primers used for cloning Cspl and making the Δcsp1/csp2 M. trichosporium OB3b strain.
Primer | Sequence (5’ to 3’)* |
---|---|
Csp1_F | GCGCATATGGGAGAGGATCCTCATGC |
Cspl_R | GCGCCATGGTCAGGCGGCGACCTTATGGC |
684AF | ATATCCCGGGTAAGGGTGAAG ACCGCCATCAG |
684AR | GATCGTCGACACGACGGACGCAACCTAAAC |
684BF | GATCGTCGACTAAGGTCGCCGCCTGAGTTC |
684BR | GATCAAGCTTCGCGCTCGCGTCCGTATTC |
1592AF | CATCAAGCTTCGGTGCGCGACATCATCCTC |
1592AR | CATCCTGCAGTGGTCGTTCCTCTCGTGTTC |
1592BF | TAATGGATCCCAGCGCGTGTCGAGCTGAAC |
1592BR | ATTAGAATTCGCGGAGCCCGCGTGGAAAG |
684TF | CACATGCAGGCGGTAGATCG |
684TR2 | CGACCAGCAGGATCATCAG |
1592TF | ACCCTTCTCACGCAATCCC |
1592TR | ACGTTGATCGGCCTCACTC |
Introduced restriction sites are underlined when relevant.