Extended Data Table 2 . Primers used for cloning Cspl and making the Δcsp1/csp2 M. trichosporium OB3b strain.

Primer	Sequence (5’ to 3’)*
Csp1_F	GCGCATATGGGAGAGGATCCTCATGC
Cspl_R	GCGCCATGGTCAGGCGGCGACCTTATGGC
684AF	ATATCCCGGGTAAGGGTGAAG ACCGCCATCAG
684AR	GATCGTCGACACGACGGACGCAACCTAAAC
684BF	GATCGTCGACTAAGGTCGCCGCCTGAGTTC
684BR	GATCAAGCTTCGCGCTCGCGTCCGTATTC
1592AF	CATCAAGCTTCGGTGCGCGACATCATCCTC
1592AR	CATCCTGCAGTGGTCGTTCCTCTCGTGTTC
1592BF	TAATGGATCCCAGCGCGTGTCGAGCTGAAC
1592BR	ATTAGAATTCGCGGAGCCCGCGTGGAAAG
684TF	CACATGCAGGCGGTAGATCG
684TR2	CGACCAGCAGGATCATCAG
1592TF	ACCCTTCTCACGCAATCCC
1592TR	ACGTTGATCGGCCTCACTC

^*Introduced restriction sites are underlined when relevant.