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. Author manuscript; available in PMC: 2016 Mar 3.
Published in final edited form as: Nature. 2015 Aug 26;525(7567):140–143. doi: 10.1038/nature14854

Extended Data Table 2 . Primers used for cloning Cspl and making the Δcsp1/csp2 M. trichosporium OB3b strain.

Primer Sequence (5’ to 3’)*
Csp1_F GCGCATATGGGAGAGGATCCTCATGC
Cspl_R GCGCCATGGTCAGGCGGCGACCTTATGGC
684AF ATATCCCGGGTAAGGGTGAAG ACCGCCATCAG
684AR GATCGTCGACACGACGGACGCAACCTAAAC
684BF GATCGTCGACTAAGGTCGCCGCCTGAGTTC
684BR GATCAAGCTTCGCGCTCGCGTCCGTATTC
1592AF CATCAAGCTTCGGTGCGCGACATCATCCTC
1592AR CATCCTGCAGTGGTCGTTCCTCTCGTGTTC
1592BF TAATGGATCCCAGCGCGTGTCGAGCTGAAC
1592BR ATTAGAATTCGCGGAGCCCGCGTGGAAAG
684TF CACATGCAGGCGGTAGATCG
684TR2 CGACCAGCAGGATCATCAG
1592TF ACCCTTCTCACGCAATCCC
1592TR ACGTTGATCGGCCTCACTC
*

Introduced restriction sites are underlined when relevant.