Figure 1.

4C-Staph formulations increased the survival of mice after S. aureus infection. BALB/c mice were immunized with 4C-Staph, 4C-Staph/MF59, 4C-Staph/alum, and respective controls (PBS, MF59, and alum) once or twice 2 weeks apart, and challenged 2 weeks after the last immunization with ~5 × 10⁸ CFU S. aureus. Animals were monitored for 7 days post-infection for health status. Graphs represent the merge of at least two separate experiments (n = 32). Statistical analyses were performed using Mantel–Cox test; survival in the groups receiving the formulated 4C-Staph vaccines were compared to their respective control. *p < 0.05.