Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 7;6:439. doi: 10.3389/fimmu.2015.00439

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Monaci, Mancini, Lofano, Bacconi, Tavarini, Sammicheli, Arcidiacono, Giraldi, Galletti, Rossi Paccani, Torre, Fontana, Grandi, de Gregorio, Bensi, Chiarot, Nuti, Bagnoli, Soldaini and Bertholet.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Persistence of IgG and CD4 T-cell responses induced by 4C-Staph formulations. BALB/c mice were immunized with 4C-Staph, 4C-Staph/MF59 and 4C-Staph/alum (n = 10), and immune responses were determined 0.5, 1, and 4 months after immunization. (A) IgG titers specific to each 4C-Staph component (Hla_H35L, EsxAB, FhuD2, and Csa1A) (n = 10–16). (B) Hla-specific neutralization titers. Number of (C) Edu⁺ proliferating and frequency of (D) cytokine⁺ CD4 T cells in response to in vitro stimulation with 4C-Staph antigens. (E) Pie charts representing polyfunctional CD4 CD44^hi T cells. (F) Frequencies of CD4 T cells expressing one or more cytokines. Graphs show mean ± SD, and are the merge of at least two separate experiments. Data were analyzed using one-way ANOVA and Tukey’s multiple comparison test, p < 0.05, *4C-Staph formulation versus its respective formulation without 4C-Staph; ^#adjuvanted-4C-Staph versus 4C-Staph alone; ^$4C-Staph/MF59 versus 4C-Staph/alum; ^†1 month or 4 months versus 0.5 month time point.