Fig. 6.

Genomic organization of xylulokinase genes from selected human or bovine-associated Bacteroidetes. The Prevotella bryantii B₁4 xylulokinase gene (GenBank accession no. EFI71375.1) was used as the query sequence in a BLASTp search of the GenBank database. Next, the Bacteroides thetaiotaomicron VPI-5482 xylose isomerase gene (BT_0793) was used as a query sequence in a BLASTp search of the GenBank database. The four organisms that possess only a xylulose kinase gene and no xylose isomerase gene are grouped in the upper half of the figure, and representative organisms that contain both genes are grouped in the lower half. The genomic context is shown for the region surrounding the xylulose kinase genes in these organisms. ORF numbers are indicated within each of the genes as derived from the genome project for each organism in the GenBank database. The small subunit (16S) ribosomal RNA genes from the organisms were aligned and a neighbour-joining tree was constructed using the CLC Genomics Workbench v3.0 software. Each alignment was re-sampled 100 times and the bootstrap values are indicated on the internal branches. The branch length is reported as the expected number of substitutions per nucleotide position. The rumen isolates are in pink and the human isolates are colour-coded as indicated in the legend based upon the reported body isolation site as listed in the Human Microbiome Projects Catalog (http://www.hmpdacc.org/).