Figure 4.

Molecular model of a flexible N-acetylated (NA) region of heparin sulphate flanked by short S domains. The model representing a long NA region in heparan sulphate (HS) was made on the basis of one of the models in the ensemble 4KHL.pdb, currently available as supplementary material to Khan et al. J. Biol. Chem. 2013, 288:27737–27751. This was a 24-mer of the heparan GlcA-GlcNAc sequence, consistent with X-ray scattering results. The two short S domains added at each end are made up of trisaccharides from the NMR structure of heparin, HPN1.pdb. This representation is an illustration, not the results of a simulation exercise. The model was kindly prepared by Professor Barbara Mulloy.