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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Jul 18;1851(10):1327–1336. doi: 10.1016/j.bbalip.2015.07.003

Fig. 1. Effect of SM depletion on the hydrolysis of labeled PC incorporated into lipoproteins.

Fig. 1

The lipoproteins were pre-labeled with dipalmitoyl [14C] PC, and an aliquot of the labeled lipoprotein was subjected to SMase treatment as described in the text. The SMase-treated and untreated lipoproteins were then incubated with recombinant HL (500 μl, 32 μg protein) for the indicated periods of time at 37 °C. The lipids were extracted, and the radioactivity in LPC, PC, and FFA was determined following their TLC separation. The results show the decrease in PC counts (%) compared to the control (incubated with no HL), and are mean ± SEM of 4 experiments. * p < 0.01 HL vs HL + SMase.