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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Jul 18;1851(10):1327–1336. doi: 10.1016/j.bbalip.2015.07.003

Fig. 5. Hydrolysis of individual molecular species of PC in the lipoproteins.

Fig. 5

The SMase-treated and native lipoproteins (400 μg protein) were incubated with 32 μg recombinant human HL for 4 h at 37 °C (Fig. 4), and the composition of molecular species of PC was analyzed by LC/MS/MS, with 17:0 PC as the internal standard. The decrease in individual PC species from the control (no HL) values was calculated. Values shown are mean ± SEM of 4 separate analyses, performed with a single batch of enzyme and 2 different batches of lipoproteins. * p< 0.05 compared to the control (no HL) values (not shown).