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. 2015 Jul 7;4(7):e246. doi: 10.1038/mtna.2015.18

Figure 1.

Figure 1

Specificity of miRNA targeting by 22-mer full-length antisense or 8-mer seed-directed oligonucleotides in cultured AGS cells. AGS cells were transfected with either the 22-mer full-length anti-miR-372 LNA/DNA antisense (AS372) or its scrambled sequence (SC372), or with the 8-mer LNA oligonucleotide targeting the nu 2–9 of miR-372 including the seed (TL372), or its negative control sequence (TLCo), at a final concentration of 10 nmol/l in the presence of Lipofectamine 2000. Cells were grown for 5 days. (a) RT-qPCR analysis of miR-372 levels; the bars represent the miR-372 levels normalized with snor25 and U6 and compared to mock treated cells (mean ± SD, n = 4). (b) Northern blot analysis of miR-372 (medium panel) and U6 (upper panel) in nondenaturing conditions; the lower panel schematizes miR-372 paired or not with the specific antimiR for each lane. (c) Nondenaturing northern blot analyses of miR-372, miR-373, miR-17-5p, and miR-93 in AGS cells transfected with either TL372, TL373, TL17, TL21, or TLCo at 10 nmol/l.