TABLE 2.

Description of the 11 VNTR markers and PCR conditions^a

Name	Tandem repeat sequence	Flanking region size (bp)	Forward primer	Reverse primer	Tm (°C)	PCR pool
TR10I	CCTGCA	118	F-AGTCTCTGCGCCTCAGGAT	GTCTGGAAAAATCCAGTGCC	53	1
TR14I	TTGATG	105	P-CTGGAAAACGTCCTGAGCAT	ACTCGGTTTGCCTGACTCAC	55	1
TR15I	GGCTGGTGCGTCT	138	F-TCGAGAGGAACACCAATGTG	TTTTGCAGACGATGTTTCCA	53	2
TR30I	AGCTACA	98	P-GCGTTACTTTGAGCGGAGTC	CACATATTCGGGTAGGTCGG	53	2
TR1II	AGGCCGAA	230	F-TGCCTGAGTACCTTTACCGG	CACCCAGCTCGACAATCAAG	59	3
TR2II	TAGTTGAGG	231b	H-GTCATAACGGGTGAGAGTGC	ACGGCCCTTGAAAGTGACTA	59	3
TR3II	TGGAGGGCT	127	N-CGTGAGGCTCTGACTTTCTG	AAATCCGGGCTGTTTATCGC	59	3
TR39II	TCGAAAA	145	P-CGGTGGACTTGAAGAACACG	CACCCTGAACTGATTGCACC	59	3
TR11II	AATTGTATCTG	136	F-GATTGGTGACGTTGCGATGA	TTGTTGCCCTACACGCTCTA	60	4
TR19II	GCTTGTA	164	H-CCCAGAAAGAATGCGGACTG	AGCAGGAGATGGAAGAGCTG	60	4
TR64II	TTGAGCT	103	P-GTTGGCGGGTATGTGTCTG	CACCACGCTTCTTCTTGCAG	60	4

^aLabeling dye abbreviations: F, 6-FAM; P, PET; H, HEX; N, NED. TRI, VNTR designed on P. syringae pv. actinidifoliorum strain ICMP 18807; TRII, VNTR designed on P. syringae pv. actinidiae biovar 3 strain ICMP 18844.

^bFlanking region size only for P. syringae pv. actinidiae biovar 3.