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. 2015 Sep 4;81(19):6558–6566. doi: 10.1128/AEM.01686-15

TABLE 3.

Proteins increased in abundance in citronellate-grown cells with postulated function in catabolism of acyclic terpenesa

Locus Gene Function Fold change in expression P value by ANOVA No. of peptides used for quantification Mol wt
PA1535 Citronellyl-CoA dehydrogenase 39.9 2.13E−05 15 42,040.8
PA1982 exaA Quinoprotein ethanol dehydrogenase 60.8 1.51E−02 36 68,122.9
PA1984 exaC NAD+-dependent aldehyde dehydrogenase 20.4 1.35E−03 32 54,892.3
PA2011 liuE 3-Hydroxy-3-methylglutaryl-CoA lyase 13.5 5.46E−04 11 31,837.1
PA2012 liuD Methylcrotonyl-CoA carboxylase, alpha subunit 17.2 2.22E−04 27 71,282
PA2013 liuC 3-Methylglutaconyl-CoA hydratase 40.5 3.76E−06 14 28,946.7
PA2014 liuB Methylcrotonyl-CoA carboxylase, beta subunit 46.7 1.31E−06 26 57,433.3
PA2015 liuA Isovaleryl-CoA dehydrogenase 24.2 2.68E−05 32 42,208.8
PA2886 atuA Protein of citronellol catabolism 92.8 2.40E−07 33 64,435.1
PA2887 atuB Putative dehydrogenase 115.6 5.71E−05 16 30,750.7
PA2888 atuC Geranyl-CoA carboxylase, beta subunit 50.3 1.40E−06 22 57,328
PA2889 atuD Citronellyl-CoA dehydrogenase 25.2 8.54E−05 32 42,714
PA2890 atuE Isohexenylglutaconyl-CoA hydratase 23.1 8.33E−05 12 27,717.4
PA2891 atuF Geranyl-CoA carboxylase, alpha-subunit 38.2 2.99E−05 34 71,747.2
PA2892 atuG Short-chain dehydrogenase 43.5 1.27E−06 17 29,630.6
PA4330 Probable enoyl-CoA hydratase/isomerase 21.2 2.49E−04 13 28,152.2
a

Selection of proteins that were increased in abundance in citronellate-grown cells compared to their abundance in octanoate-grown cells and with a proposed function in the catabolism of citronellol. Proteins of the Atu and Liu gene clusters are in bold. For a complete overview of all proteins identified in our proteomics survey, see Data Set S2 in the supplemental material.