TABLE 3.
Locus | Gene | Function | Fold change in expression | P value by ANOVA | No. of peptides used for quantification | Mol wt |
---|---|---|---|---|---|---|
PA1535 | Citronellyl-CoA dehydrogenase | 39.9 | 2.13E−05 | 15 | 42,040.8 | |
PA1982 | exaA | Quinoprotein ethanol dehydrogenase | 60.8 | 1.51E−02 | 36 | 68,122.9 |
PA1984 | exaC | NAD+-dependent aldehyde dehydrogenase | 20.4 | 1.35E−03 | 32 | 54,892.3 |
PA2011 | liuE | 3-Hydroxy-3-methylglutaryl-CoA lyase | 13.5 | 5.46E−04 | 11 | 31,837.1 |
PA2012 | liuD | Methylcrotonyl-CoA carboxylase, alpha subunit | 17.2 | 2.22E−04 | 27 | 71,282 |
PA2013 | liuC | 3-Methylglutaconyl-CoA hydratase | 40.5 | 3.76E−06 | 14 | 28,946.7 |
PA2014 | liuB | Methylcrotonyl-CoA carboxylase, beta subunit | 46.7 | 1.31E−06 | 26 | 57,433.3 |
PA2015 | liuA | Isovaleryl-CoA dehydrogenase | 24.2 | 2.68E−05 | 32 | 42,208.8 |
PA2886 | atuA | Protein of citronellol catabolism | 92.8 | 2.40E−07 | 33 | 64,435.1 |
PA2887 | atuB | Putative dehydrogenase | 115.6 | 5.71E−05 | 16 | 30,750.7 |
PA2888 | atuC | Geranyl-CoA carboxylase, beta subunit | 50.3 | 1.40E−06 | 22 | 57,328 |
PA2889 | atuD | Citronellyl-CoA dehydrogenase | 25.2 | 8.54E−05 | 32 | 42,714 |
PA2890 | atuE | Isohexenylglutaconyl-CoA hydratase | 23.1 | 8.33E−05 | 12 | 27,717.4 |
PA2891 | atuF | Geranyl-CoA carboxylase, alpha-subunit | 38.2 | 2.99E−05 | 34 | 71,747.2 |
PA2892 | atuG | Short-chain dehydrogenase | 43.5 | 1.27E−06 | 17 | 29,630.6 |
PA4330 | Probable enoyl-CoA hydratase/isomerase | 21.2 | 2.49E−04 | 13 | 28,152.2 |
Selection of proteins that were increased in abundance in citronellate-grown cells compared to their abundance in octanoate-grown cells and with a proposed function in the catabolism of citronellol. Proteins of the Atu and Liu gene clusters are in bold. For a complete overview of all proteins identified in our proteomics survey, see Data Set S2 in the supplemental material.