Lipid development in wild-type and developmental mutants of M. xanthus. All lipid subspecies of the wild type and the esg and fruA mutants during development were investigated, and their abundances were compared. White bars, lipid profiles in wild-type cells; gray and black bars, lipid profiles in esg and fruA mutant cells, respectively. The relative amounts of the individual lipid subspecies at 0 h were set equal to 100. Error bars show standard deviations from triplicate experiments. (A) Ceramides; (B) phosphatidylethanolamines; (C) phosphatidylglycerols; (D) diacylglycerols; (E) triacylglycerols; (F) PE(P-iso-15:0/iso-15:0); (G) 1-iso-15:0-alkyl-2,3-di-iso-15:0-acyl glycerol.