Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Enzymatic properties of wild-type PG8fn and its mutants (50 μg/ml). (A) Temperature-dependent activity profiles determined at pH 5.5 for 10 min; (B) enzyme inactivation assay at different temperatures for 30 min; (C and D) time courses of thermal inactivation at 50°C and 55°C, respectively. The enzymes with and without deglycosylation with endo H are marked with solid and dashed lines, respectively. Each data point represents an average from three independent experiments.
