TABLE 1.
Primers used in this study
| Primer | Sequence (5′→3′)a |
|---|---|
| D7A-F | CTCCCCGCCAAGCGCGCGAGCTGCACCTTTACCGCTGCTGCCT |
| D25A-F | CATCACCCTCAAGGCTATCACCGTCCCCG |
| D25A-R | GGGGACGGTGATAGCCTTGAGGGTGATGGT |
| E73A-F | CATCCACGTCGCTGGCGCCCCCGGCCATGT |
| E73A-R | CGGGGGCGCCAGCGACGTGGATGTTGTT |
| E132A-F | TCAACGGCGCTGCTAACCTCGGAGTGTAC |
| E132A-R | CCGAGGTTAGCAGCGCCGTTGATGCTGAAG |
| D138A-F | CTCGGAGTGTACGCTGTCCACCTCGACAAC |
| D138A-R | TCGAGGTGGACAGCGTACACTCCGAGGTTT |
| D244A-F | CTCCGTGTCGGCTGTCGAGTACTCGGGCA |
| D244A-R | GAGTACTCGACAGCCGACACGGAGCCCTTG |
| E246K-F | TGTCGGACGTCAAGTACTCGGGCATCACAC |
| E246K-R | ATGCCCGAGTACTTGACGTCCGACACGGAG |
| E267K-F | AGCAGGACTACAAGAACGGCTCGCCCACCG |
| E267K-R | GGCGAGCCGTTCTTGTAGTCCTGCTCGATG |
| D299R-F | CGGGCGCCACCAGAGTCTACATCCTCTGCG |
| D299R-R | AGGATGTAGACTCTGGTGGCGCCCGACTTG |
a
Mutation sites are underlined.