FIG 1.
Plasmid copy number and antibiotic production by S. clavuligerus ΔclaR::aac. (A) Relative amount of DNA measured by qPCR of the genes indicated above. DNA samples were obtained from S. clavuligerus claR::aph pSCLlow (black columns), S. clavuligerus ΔclaR::aac (gray columns), and S. clavuligerus oppA2::aph pSCLlow (white columns). The relative amounts of DNA shown for the three mutant strains were determined by comparison to the amounts obtained with wild-type S. clavuligerus. (B) Production of clavulanic acid (left panel), holomycin (central panel), and cephamycin C (right panel) by S. clavuligerus ATCC 27064 (white circles), S. clavuligerus ΔclaR::aac (black circles), S. clavuligerus ΔclaR::aac(pMS83) (black squares), and S. clavuligerus ΔclaR::aac(pMS83-claR) (white squares).