FIG 2.
The genetic location of Ptrc1O-eyfp influences gene expression. Shown are normalized fluorescence intensities ± standard deviations and growth curves over a time course of 8 days for mutants expressing Ptrc-eyfp in NSC1, NSC2, NSP1, and a broad-host-range vector containing the RSF1010 replicon. Cells were grown in BG11 medium (39) supplemented with kanamycin (10 μg/ml) as needed under continuous white light at 30 μmol m−2 s−1 at 30°C. Cultures were grown in TPP tissue culture-treated 12-well plates (Sigma-Aldrich).