TABLE 2.

Summary of 16S/18S rRNA amplicon sequence data and derived diversity parameters for 23 oil sands outcrop samples and 4 enrichments

Samplea	SRR accession no.	No. of quality control reads	%			No. of quality control reads for prokaryotes	No. of OTUs		Shannon's H indexb	Good's coverage (%)	No. of prokaryotic taxa
			Archaea	Bacteria	Eukaryota		Prokaryotes (5% cutoff)	Total (Chao1 indexb)
HR_M	1784302	8,797	0.54	97.25	2.23	8,601	952	875	4.72	95	310
SR2_C	621492	8,594	1.57	96.90	1.56	8,460	823	677	4.16	95	297
HR2_C	621565	9,575	1.31	97.54	1.17	9,463	858	561	4.34	95	287
SR1_C	621490	6,207	0.11	97.56	2.29	6,065	623	535	4.50	95	221
SR2_S	621625	4,318	1.36	94.00	4.63	4,118	925	1,063	5.42	88	306
SR1_S	621491	6,980	0.46	98.76	0.76	6,927	672	682	4.41	95	200
HR2_S	621566	12,771	0.69	99.14	0.20	12,746	906	762	3.93	97	277
HR1_S	621563	10,923	1.02	97.74	1.25	10,787	877	755	4.30	96	291
HR1_C	621559	5,568	1.17	96.94	1.89	5,463	528	455	3.59	95	238
OC2007	621597	5,524	2.51	95.99	1.47	5,443	674	757	4.52	94	282
HC_M	1784301	5,564	16.38	80.09	3.54	5,367	260	237	3.22	98	147
SC3_C	621629	3,974	0.23	70.93	28.84	2,828	353	458	4.03	94	148
SC3_S	621626	7,267	0.03	71.65	28.32	5,209	449	419	3.99	96	150
SC2_S	621628	6,217	0.03	79.84	20.12	4,966	232	354	2.74	98	112
SC2_C	621627	4,046	0	76.14	23.85	3,081	165	222	2.86	97	87
HC1_C	621560	3,349	1.46	82.01	16.54	2,795	188	209	3.30	97	108
SC1_S	621624	2,405	0.63	62.66	36.72	1,522	149	247	3.28	95	86
HC2_S	621561	5,672	0	86.49	13.50	4,906	285	269	3.37	97	90
HC3_S	621567	8,113	0.01	90.70	9.29	7,359	244	187	3.12	99	84
HC1_S	621562	8,663	0.04	86.86	13.09	7,529	302	186	3.35	98	115
HC3_C	629322	1,490	28.26	70.39	1.34	1,470	173	307	3.67	94	118
HC2_C	629219	6,498	10.05	85.90	4.03	6,236	376	369	4.21	98	226
SC1_C	629218	6,772	11.95	86.91	1.17	6,693	357	308	3.96	98	225
Oxic_4	629342	2,002	0	92.00	7.99	1,842	243	390	3.87	93	124
Oxic_55	631218	4,406	0.11	99.73	0.16	4,399	331	162	2.06	98	83
Anoxic_23	631221	5,277	49.67	50.09	0.25	5,264	152	326	2.68	96	150
Anoxic_60	631220	4,561	45.88	52.44	1.67	4,485	225	257	2.99	98	128
Sum		171,101				159,487
Total							4,750				723
Avg			6.31	85.48	8.20					96

^aThe samples are described in Table 1; Oxic_4, Oxic_55, Anoxic_23, and Anoxic_60 are enrichments of OC2007 at the indicated temperatures (4, 55, 23, and 60°C, respectively) in air (oxic) or in H₂-CO₂ (anoxic). The samples are listed from top to bottom in the same order as the branches in the dendrogram in Fig. 3A.

^bBiodiversity indices were calculated using a normalized number of 1,490 reads (observed for HC3_C).