TABLE 3.

Statistical summary of oil sands outcrop metagenomes for cliff (HC_M) and river water (HC_R) samples and summary of sequence counts for key metabolic genes relative to rpoB^a

Feature	Database searchb	Result for sample:
		HC_M	HR_M
SRA accession no.		SRX327733	SRX327734
No. of base pairs sequenced after quality control		423,985,802	340,253,804
No. of reads after quality control		548,460	436,518
Average read length (bp)		773	779
No. of 16S rRNA gene reads		420	278
Bacteria (%)		94.52	99.28
Archaea (%)		0.24	0
No. of 18S rRNA gene reads		139	15
Fungi (%)		63.59	13.33
Other eukaryotes (%)		35.25	86.67
Pathway or enzyme class
Dioxygenases
Dioxygenase	PF00775	2.95	2.33
Catechol dioxygenase	PF04444	1.42	0.8
Protocatechuate 3,4-dioxygenase	PF12391	1.15	0.36
Cytochrome P450 monooxygenases
CYP52	FCPD	13.2	4.08
CYP53	FCPD	15.82	4.52
CYP504	FCPD	10.42	4.52
Manganese peroxidases	fPoxDB	0.44	0.51
Laccases	blast	9.82	8.09
Anaerobic degradation of aromatics
Benzylsuccinate synthase (bssABCDEF)	HMM	0	0.07
Ethylbenzene dehydrogenase (EB_dh)	PF09459	0	0.22
Class I Benzoyl-CoA reductase (brcABCD)	HMM	0	0.29
Class II Benzoyl-CoA reductase (bamBCDEFGHI)	HMM	0	0.22
CH4 cyclec
Particulate methane monooxygenase (pmoA)	PF14100	0.38	0.73
Soluble methane monooxygenase (mmoX)	FunGene	0.05	0
Sulfur oxidation and assimilation
soxY	PF13501	1.04	1.53
soxZ	PF08770	0.93	0.66

^aA total of 1,833 and 1,372 hits of the rpoB gene were obtained for HC_M and HR_M, respectively.

^bAbbreviations for database searches: PF, HMM search against the Pfam seed database; FunGene, HMM search against FunGene database; HMM, HMM search against customer-created functional gene HMM; FCPD, blast search against the Fungal Cytochrome P450 Database; fPoxDB, blast search against the Fungal Peroxidase Database; blast, blast search against customer-created functional gene database. PF numbers, Pfam protein accession numbers.

^cNo genes for methanogenesis (e.g., mcrA) were found.