FIG 1.

Effect of temperature on inactivation of RV serotype G1 strains. RV stock (10⁶ PFU/ml) in cell culture medium (MEM) was processed under pressures ranging from 200 MPa to 450 MPa held for 2 min at either 4°C or 20°C. The surviving viruses were determined by plaque assay. Data are the means of three replicates. Error bars represent standard deviations. (A) Effect of temperature on strain Wa; there was no significant difference in viral reduction between 4 and 20°C (P > 0.05). (B) Effect of temperature on strain Ku; there was a significant difference in reduction between 4 and 20°C (P < 0.05). (C) Effect of temperature on strain K8; there was a significant difference in reduction between 4 and 20°C (P < 0.05).