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Effect of temperature on inactivation of RV serotype G2 strain S2. RV stock (106 PFU/ml) in cell culture medium (MEM) was processed under pressures ranging from 200 MPa to 450 MPa held for 2 min at either 4°C or 20°C. The surviving viruses were determined by plaque assay. Data are the means of three replicates. Error bars represent standard deviations. Viral reduction was significantly different between the initial temperatures of 4°C and 20°C (P < 0.05).
