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Effect of HPP on RV proteins. Purified RV TLPs and DLPs were pressurized at 200, 400, and 600 MPa at 4°C for 2 min. The structural proteins of untreated and treated RVs were analyzed by 15% SDS-PAGE followed by Coomassie blue staining. (A) SDS-PAGE of 2 μg of total RV Wa TLPs. (B) SDS-PAGE of 2 μg of total RV SA-11 TLPs. (C) SDS-PAGE of 1.0 μg of total RV Wa DLPs. (D) SDS-PAGE of 1.0 μg of total RV SA-11 DLPs.
