Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Hpr phosphorylation by HprK/P. Hpr-His6 (20 μM) was mixed with HprK/P-His6 (1 μM) in the presence of 2 mM ATP or 20 mM FBP. The reaction products were analyzed by both nondenaturing 12% PAGE and SDS-PAGE. Lane 1, Hpr; lane 2, Hpr and HprK/P; lane 3, Hpr, HprK/P, ATP, and FBP; lane 4, Hpr, HprK/P, and FBP; lane 5, Hpr, HprK/P, and ATP.
