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. 2015 Sep 4;81(19):6548–6557. doi: 10.1128/AEM.01549-15

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FIG 3 — EMSAs to detect binding of CcpA_Bt and YvoA_Bt to their targets. DNA fragments were detected by staining. (The concentrations of CcpA_Bt and YvoA_Bt are indicated in the labels above each panel.) (A) Analysis of YvoA_Bt binding to PchiB (158 bp). A total of 0.05 μM PchiB was mixed with various concentrations of YvoA_Bt. (B) Analysis of CcpA_Bt binding to PchiB. A total of 0.05 μM PchiB was mixed with various concentrations of CcpA_Bt. (C to E) Analysis of CcpA_Bt binding to Pcre (40 bp). (C) A total of 0.1 μM Pcre was mixed with various concentrations of CcpA_Bt. (D) A total of 0.1 μM was Pcre was mixed with various concentrations of CcpA_Bt in the presence of 4 μM Hpr. (E) A total of 0.1 μM Pcre was mixed with various concentrations of CcpA_Bt in the presence of 4 μM Hpr-Ser₄₅-P.