TABLE 1.
Culture doubling times for adapted cellsa
| Strain | MeOH |
MeOH + acetate |
Acetate |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Doubling time (h) (±SD) | P vs parent | P vs ΔhdrABC | Doubling time (h) (±SD) | P vs parent | P vs ΔhdrABC | Doubling time (h) (±SD) | P vs parent | P vs ΔhdrABC | |
| Parent | 8.5 ± 0.17 | 1 | 0.0000 | 9.4 ± 0.28 | 1 | 0.0046 | 44.0 ± 2.80 | 1 | 0.0000 |
| att::hdrABC* | 8.4 ± 0.35 | NS | 0.0001 | 9.7 ± 0.16 | NS | NS | 47.6 ± 2.64 | NS | 0.0003 |
| ΔhdrABC att::hdrABC* | 9.1 ± 0.14 | 0.0012 | 0.0010 | 10.3 ± 0.48 | NS | NS | 58.0 ± 3.05 | 0.0002 | NS |
| ΔhdrABC | 9.9± 0.24 | 0.0000 | 1 | 10.1 ± 0.20 | 0.0046 | 1 | 61.8 ± 2.94 | 0.0000 | 1 |
a
Cells were adapted to methanol for 25 generations. Data were collected from five biological replicates (n = 5). Significance (P values) was determined by unpaired Student t tests. NS, not significant (P > 0.01).