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The PCP of Spt5 is important for in vivo function. (A) Growth of yeast strains following plasmid shuffle in the SPT4 background. (B) Growth in the spt4Δ background. Note that Q25 promoted the emergence of rare suppressor-like colonies. (C) Far-UV CD spectra taken from purified mutant K1L1 proteins of the WT and Q20 through Q25 (with the color code indicated).
