FIG 2.

Pancreatic progenitor tracing and Men1 disruption in pancreatic progenitors define the derivation of NPG cells. (A) Schematic illustration of the two mouse models used for lineage tracing of pancreatic progenitors and pancreatic endocrine progenitors, respectively. (B) Representative IF staining for gastrin and enhanced YFP (eYFP) in the neonatal pancreases of control R26^eYFP mice, Ptf1a-Cre⁺-Rosa26^eYFP mice, and Ngn3^wt/tTA-tetO-Cre⁺-R26^eYFP mice. Gastrin-expressing cells express the genetic tracer enhanced YFP. (C) IF staining for menin and gastrin in the neonatal pancreases of PancEndoMen1 KO mice. Bars = 20 μm.