FIG 4.

Screen for Bicc1 SAM polymer mutants. (A) Bicc1 SAM mutant collection. Shading is as for Fig, 3A. Individual electrostatic patches (patches A to F) at the protein surface were replaced by alanines. (B, C) Positions of mutations in the Bicc1 SAM dimer model (B) and on the ML surface and EH surface (C). (D) Table summarizing, for each amino acid patch, the average number of H bonds per time frame during the MD. (E and F) Pulldown of the WT, point mutants, or 8-fold excess bpk mutant HA-tagged Bicc1 from HEK293T cell extracts by glutathione-Sepharose beads coated with a recombinant GST control or GST-Bicc1 SAM. Five percent of total cell extracts were loaded as input.