FIG 1.

Induction of Nur77 expression by multiple hypertrophic stimuli. (A) NRVMs were treated with hypertrophic stimuli at the indicated concentrations for 1 h. Nur77 expression was determined by Northern blotting. (B) Time-dependent effect of ISO (10 μmol/liter) on Nur77 expression as determined by Northern blotting. (C) Dose-dependent effect of ISO on Nur77 expression as determined by Northern blotting 1 h after ISO stimulation. (D) Adult mice were treated with a single intraperitoneal injection of ISO (1 mg/kg), and their hearts were then harvested at the indicated time points for determining Nur77 expression by qRT-PCR. *, P < 0.05 versus untreated control or time at 0 h. The data represent 3 independent experiments. (E) Myocytes were pretreated with vehicle, various kinase inhibitors, or β-AR antagonist for 1 h and then treated with ISO (10 μmol/liter) for 1 h, and Nur77 expression was determined by Northern blotting.