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. 2015 Sep 4;35(19):3312–3323. doi: 10.1128/MCB.00229-15

FIG 2.

FIG 2

ISO increases Nur77 protein expression in NRVMs. (A) Myocytes were treated with ISO (10 µmol/liter) for the indicated times. Nur77 expression was determined by Western blotting (n = 3). *, P < 0.05 versus time zero. (B) Myocytes were treated with indicated concentrations of ISO for 3 h. Nur77 expression was determined by Western blotting (n = 3). *, P < 0.05 versus ISO dose at time zero. (C) Myocytes were treated with ISO (10 µmol/liter) for 3 h and then fixed and sequentially incubated with anti-Nur77 polyclonal antibody and FITC-conjugated donkey anti-rabbit antibody. Images were visualized using an epifluorescence microscope. (D) Myocytes were treated with ISO (10 µmol/liter) or ET-1 (100 nmol/liter) for 3 h. Nuclear (Nucl) and cytoplasmic (Cyto) fractions were isolated and subjected to Western blot analysis to determine expression of Nur77. (E) Mice were administered ISO with a mini-osmotic pump (60 mg/kg/day). Hearts were harvested at the indicated time points, and Nur77 expression was determined by Western blotting (n = 3). *, P < 0.05 versus day 0.